A novel thermo-pH responsive and fluorescent based-p(ANIPAM) nanoparticles (NPs) were prepared by free-radical precipitation method. We evaluated the internalization and cytotoxic effect of the NPs in vitro model on Arpe-19 and MDA-MB-231 breast cancer cell line. In addition we research the role of low molecular weight chitosan (CH) on the lower critical solution temperature (LCST) of the NPs.

Structural characterization of native and modified p(NIPAM) NPs was performed using (FTIR). Optical activity was elucidated by fluorescence (FL) spectroscopy. Evolution of the morphology and size of the NPs were analyzed using images of Atomic Force Microscopy (AFM) and of Dynamic Light Scattering measurements (DLS). It was found that it is possible to modulate particle size as a function of Low molecular weight CH concentration. Fluorescence microscopy images demonstrate successful high concentration NPs internalization in breast cancer cell lines. MTT reduction method shows low cytotoxic effect in MDA-MB-231 cell lines at moderate concentration of NPs. Interactions assays demonstrated the successfully internalization in great concentration of NPS PC3F in vitro model on MDA-MB-231 breast cancer cell lines.

Physicochemical profile (shape, size and LSCT transition) of these nanoparticles is interesting for the potential application as thermo-pH-controlled platform in drug delivery in breast cancer therapy.